FastQCFastQC Report
Sat 1 Jul 2023
HGNH2AFX5_n02_as6-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGNH2AFX5_n02_as6-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5084197
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAAATGGCTGATTTTGATTGTTTATGTTTTGTGTGATGATTTTACA71140.13992376770609005No Hit
CGGCAAAATGTTAGTGCAGGAAAGCGGGAAGGAATAAGAAGCAACTAAAC69730.13715046840238487No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC69610.13691444292972912Illumina Single End PCR Primer 1 (100% over 50bp)
TGCGGCAAAATGTTAGTGCAGGAAAGCGGGAAGGAATAAGAAGCAACTAA68070.1338854493639802No Hit
GCGGCAAAATGTTAGTGCAGGAAAGCGGGAAGGAATAAGAAGCAACTAAA59690.11740300385685291No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG53260.10475597228038175No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)