FastQCFastQC Report
Sat 1 Jul 2023
HGNH2AFX5_n02_as6-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGNH2AFX5_n02_as6-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8759154
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAAATGGCTGATTTTGATTGTTTATGTTTTGTGTGATGATTTTACA183400.20938095163071682No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC180170.20569338089043757Illumina Single End PCR Primer 1 (100% over 50bp)
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA147760.16869209058317733No Hit
TGGTGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCAT115800.1322045485214668No Hit
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC113100.12912205904816837Illumina Single End PCR Primer 1 (100% over 49bp)
GGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGAT106110.12114183630062902No Hit
TGGGTGGAGGACAATTTTTATTATATTTCATCTAATAGCAATAGGATATG104740.11957775830862205No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96050.10965670885567258No Hit
GCGGCAGTATTGAGACCATGAGAGTAGCAAACGTAAGTCTAAAGGTTGTT95730.10929137676994832No Hit
GGTGGAGGACAATTTTTATTATATTTCATCTAATAGCAATAGGATATGAC90720.10357164630282788No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)