FastQCFastQC Report
Sat 1 Jul 2023
HGNH2AFX5_n02_as6-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGNH2AFX5_n02_as6-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5910135
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA134060.226830689992699No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC113900.1927197940486977Illumina Single End PCR Primer 1 (100% over 50bp)
TGGTGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCAT106790.1806896119970187No Hit
TGCGGAAATGGCTGATTTTGATTGTTTATGTTTTGTGTGATGATTTTACA101390.1715527648691612No Hit
GGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGAT93010.15737373173370828No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG89380.1512317400533152No Hit
GCGGCAGTATTGAGACCATGAGAGTAGCAAACGTAAGTCTAAAGGTTGTT86540.1464264352675531No Hit
TGGGTGGAGGACAATTTTTATTATATTTCATCTAATAGCAATAGGATATG71860.1215877471495998No Hit
TGTGGAAGACATAGATGGTATTTGTTTTGCATTTACGGCACCGGATGCGG60080.10165588434105143No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)