FastQCFastQC Report
Sat 1 Jul 2023
HGNH2AFX5_n02_as6-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGNH2AFX5_n02_as6-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5093865
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC842091.6531454995371884Illumina Single End PCR Primer 1 (100% over 50bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC257070.5046659069292179Illumina Single End PCR Primer 1 (100% over 49bp)
TGCGGAAATGGCTGATTTTGATTGTTTATGTTTTGTGTGATGATTTTACA179340.3520705790200565No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGT128110.25149861647295324Illumina Single End PCR Primer 1 (96% over 30bp)
TGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG127250.24981031103101475Illumina Single End PCR Primer 1 (100% over 35bp)
TCGGAAGAGCGTCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTA116920.2295310142691257Illumina Single End PCR Primer 1 (96% over 29bp)
TAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT105660.20742599185490782Illumina Single End PCR Primer 1 (100% over 37bp)
TGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA99870.1960593773097638Illumina Single End PCR Primer 1 (100% over 34bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC98160.19270239788451404Illumina Single End PCR Primer 1 (100% over 49bp)
GAGCGTCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAA93500.18355413816424265Illumina Single End PCR Primer 1 (100% over 27bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTG91040.1787247993419535Illumina Single End PCR Primer 1 (97% over 36bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAG88850.17442550990259853Illumina Single End PCR Primer 1 (96% over 30bp)
TGTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT88310.17336541113673018Illumina Single End PCR Primer 1 (100% over 37bp)
CGTCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG86850.17049921817716016Illumina Single End PCR Primer 1 (100% over 30bp)
GTCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT86300.16941948795266462Illumina Single End PCR Primer 1 (100% over 31bp)
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA83530.16398157391293253No Hit
TCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGT82260.16148837866727916Illumina Single End PCR Primer 1 (97% over 35bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC82190.16135095845688882Illumina Single End PCR Primer 1 (100% over 49bp)
TCGGAAGAGCGTCGTGTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGG81950.16087980344983624Illumina Single End PCR Primer 1 (96% over 29bp)
TAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA81900.16078164615670026Illumina Single End PCR Primer 1 (100% over 36bp)
GAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAA81340.15968228447357755Illumina Single End PCR Primer 1 (96% over 29bp)
CGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGA79310.1556970983722576Illumina Single End PCR Primer 1 (100% over 29bp)
GTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG73890.1450568477963197Illumina Single End PCR Primer 1 (100% over 30bp)
AGCGTCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAG71490.14034529772579368Illumina Single End PCR Primer 1 (100% over 28bp)
GTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA70940.13926556750129812Illumina Single End PCR Primer 1 (100% over 34bp)
TGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGACTGAAGTGGGG70590.1385784664493464No Hit
TAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT69210.13586932515879396Illumina Single End PCR Primer 1 (100% over 39bp)
AGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAA67340.13219824239550912Illumina Single End PCR Primer 1 (96% over 28bp)
GAGCGTCGTGTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGA62980.12363892643405353Illumina Single End PCR Primer 1 (100% over 29bp)
TGTAGGGAAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT61620.12096904806075544Illumina Single End PCR Primer 1 (100% over 39bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGATCGGAAGAGCGTCGTGTAGGG59640.11708201925257147Illumina Single End PCR Primer 1 (96% over 28bp)
TCGGAAGAGCGTCGTGTAGGGAAAGAGATCGGAAGAGCGTCGTGTAGGGA58990.11580597444180402Illumina Single End PCR Primer 1 (96% over 27bp)
GTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT57750.11337167357203225Illumina Single End PCR Primer 1 (100% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTG57030.11195820855087445Illumina Single End PCR Primer 1 (96% over 31bp)
GTCGTGTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT56230.1103876918606991Illumina Single End PCR Primer 1 (100% over 33bp)
TGGTGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCAT56040.11001469414678244No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTA53210.10445899135528719Illumina Single End PCR Primer 1 (96% over 31bp)
AGCGTCGTGTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG52920.10388967905509863Illumina Single End PCR Primer 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)