FastQCFastQC Report
Sat 1 Jul 2023
HGNH2AFX5_n02_as6-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGNH2AFX5_n02_as6-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624978
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC84450.32171698200899207Illumina Single End PCR Primer 1 (100% over 50bp)
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA49960.19032540463196262No Hit
TAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT41180.1568775052590917Illumina Single End PCR Primer 1 (100% over 37bp)
TGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG40950.156001307439529Illumina Single End PCR Primer 1 (100% over 35bp)
TGGTGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCAT40720.15512510961996634No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38590.1470107558996685No Hit
TGTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT37160.14156309119543098Illumina Single End PCR Primer 1 (100% over 37bp)
GGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGAT37150.14152499563805868No Hit
TAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT36590.1393916444252104Illumina Single End PCR Primer 1 (100% over 39bp)
TGCGGAAATGGCTGATTTTGATTGTTTATGTTTTGTGTGATGATTTTACA36240.13805829991718027No Hit
TGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA30730.11706764780504827Illumina Single End PCR Primer 1 (100% over 34bp)
GCGGCAGTATTGAGACCATGAGAGTAGCAAACGTAAGTCTAAAGGTTGTT28250.10761994957672026No Hit
TAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA27670.10541040724912742Illumina Single End PCR Primer 1 (100% over 36bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGT27110.10327705603627917Illumina Single End PCR Primer 1 (96% over 30bp)
TGGTGAGAGAAAATAATAAAGGTCTAATAAGTATTATGTGAAAAAAATGT26270.10007702921700677No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)