FastQCFastQC Report
Sat 1 Jul 2023
HGNH2AFX5_n02_as6-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGNH2AFX5_n02_as6-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6756928
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA165620.24511138789698514No Hit
TGGTGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCAT136430.2019112827604497No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC134920.19967653939778549Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG128810.19063396857270049No Hit
GGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGAT122280.18096981350104663No Hit
GCGGCAGTATTGAGACCATGAGAGTAGCAAACGTAAGTCTAAAGGTTGTT101870.15076377904278393No Hit
TGCGGAAATGGCTGATTTTGATTGTTTATGTTTTGTGTGATGATTTTACA87580.12961511503452458No Hit
TGGGTGGAGGACAATTTTTATTATATTTCATCTAATAGCAATAGGATATG82960.12277768832226715No Hit
TAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT81730.12095733445731552Illumina Single End PCR Primer 1 (100% over 37bp)
TGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG78930.11681343948018981Illumina Single End PCR Primer 1 (100% over 35bp)
TGGTGGTAGTGTAATGTATGATATCCGTTGGTTTTGGTTTCGGTTGTGAA74780.11067159513909279No Hit
TGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA73750.10914723377250728Illumina Single End PCR Primer 1 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)