FastQCFastQC Report
Sat 1 Jul 2023
HGNH2AFX5_n02_as6-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGNH2AFX5_n02_as6-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7304414
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC286160.39176311747937614Illumina Single End PCR Primer 1 (100% over 50bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC106710.14608974792502177Illumina Single End PCR Primer 1 (100% over 49bp)
TGCGGAAATGGCTGATTTTGATTGTTTATGTTTTGTGTGATGATTTTACA81870.11208291315360822No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)