FastQCFastQC Report
Sat 1 Jul 2023
HGNH2AFX5_n01_as6-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGNH2AFX5_n01_as6-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5093865
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG801351.5731669370900092TruSeq Adapter, Index 4 (100% over 49bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAT236670.46461773132974665TruSeq Adapter, Index 4 (100% over 48bp)
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTC133260.26160881766595695Illumina Single End PCR Primer 1 (96% over 30bp)
CACTCTTTCCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT126420.24818089996495787Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCT121530.23858111669626111Illumina Single End PCR Primer 1 (96% over 29bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGT107470.2109792858664295Illumina Single End PCR Primer 1 (96% over 31bp)
CACTCTTTCCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA105120.20636589308903946TruSeq Adapter, Index 2 (97% over 36bp)
ACACTCTTTCCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC99890.1960986402270182Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CACTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTCC99790.19590232564074628Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTC98920.19419438874018058Illumina Single End PCR Primer 1 (96% over 30bp)
CACTCTTTCCCTACACGACGCTCAGATCGGAAGAGCACACGTCTGAACTC94800.1861062277857776Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTG93260.18308298315719007Illumina Single End PCR Primer 1 (96% over 28bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAT92680.18194435855681296TruSeq Adapter, Index 4 (100% over 48bp)
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG87320.17142189673263816Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCTTTCCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA87210.17120595068773906TruSeq Adapter, Index 2 (97% over 36bp)
CTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGA86260.16934096211815586Illumina Single End PCR Primer 1 (96% over 27bp)
CACTCTTTCCCTACACGACAGATCGGAAGAGCACACGTCTGAACTCCAGT85740.16832012626954188Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACACTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTC84520.1659250883170245Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACACTCTTTCCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG81900.16078164615670026TruSeq Adapter, Index 2 (97% over 35bp)
ACACTCTTTCCCTACACGACGCTCAGATCGGAAGAGCACACGTCTGAACT80650.1583277138283013Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCA80130.15730687797968734Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAT75920.14904203389763962TruSeq Adapter, Index 4 (100% over 48bp)
ACACTCTTTCCCTACACGACAGATCGGAAGAGCACACGTCTGAACTCCAG74290.14584210614140736Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACTCTTTCCCTACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC70760.13891220124600867Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCTTTCCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC68900.135260749941351TruSeq Adapter, Index 4 (100% over 38bp)
CTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTCCAG68720.13490738368606156Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCTTTCCCTACACGACGCTCAGATCGGAAGAGCACACGTCTGAACTCCA64340.1263088048073516Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAA61620.12096904806075544Illumina Single End PCR Primer 1 (96% over 26bp)
CTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAAC61450.12063531326409317Illumina Single End PCR Primer 1 (96% over 25bp)
CTTTCCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC61430.12059605034683879TruSeq Adapter, Index 4 (100% over 38bp)
ACACTCTTTCCCTACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA58090.11403914316535677Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGT57380.11264530960282615Illumina Single End PCR Primer 1 (96% over 31bp)
CTCTTTCCCTACACGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA55830.10960243351561144Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCT55350.10866012350150622Illumina Single End PCR Primer 1 (96% over 29bp)
CTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC51020.10015970191593221Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGA50970.10006154462279623Illumina Single End PCR Primer 1 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)