FastQCFastQC Report
Sat 1 Jul 2023
HGNH2AFX5_n01_as6-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGNH2AFX5_n01_as6-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624978
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG69160.2634688747867601TruSeq Adapter, Index 3 (100% over 49bp)
CACTCTTTCCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA42040.16015372319310867TruSeq Adapter, Index 3 (100% over 36bp)
CACTCTTTCCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT41710.15889656979982308Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCTTTCCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG37700.14362025129353465TruSeq Adapter, Index 3 (100% over 38bp)
CTCTTTCCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA37520.14293453126083341TruSeq Adapter, Index 3 (100% over 36bp)
ACACTCTTTCCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC31130.1185914700999399Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTC29090.11081997639599266Illumina Single End PCR Primer 1 (96% over 30bp)
ACACTCTTTCCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT28120.10712470733088048TruSeq Adapter, Index 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)