FastQCFastQC Report
Sat 1 Jul 2023
HGNH2AFX5_n01_as6-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGNH2AFX5_n01_as6-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6756928
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG110600.16368385159646515TruSeq Adapter, Index 2 (100% over 49bp)
CACTCTTTCCCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA81950.12128292620551825TruSeq Adapter, Index 2 (100% over 36bp)
CACTCTTTCCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC79400.11750902185135019Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACACTCTTTCCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC74220.10984281614366766Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)