FastQCFastQC Report
Wed 15 Aug 2018
HGN57BGX7_n01_6afp.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN57BGX7_n01_6afp.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGGAAAAAAAAAAAAAAAACAGCGAAAAAAAAAAACGGACGAGCACCC112.5No Hit
CGGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC112.5Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGGGGGGGGGAAAAAAAAAAGATCGGAAGGGAACACGTCTGAACTACA112.5No Hit
CCGGGAAAAAAAAAAAAAAAGGAAAAAAAAAAAAAAAAAAAGAAAGGCAA112.5No Hit
CCGGGGATAGAAAAAAAAAAAGAAAGATTGAACGAGCGTAAGACGGACCT112.5No Hit
GGTGCAAAAAAAAAAAAGGAAACCAGAAAAAAAAAAAGCAAACGGCGGAC112.5No Hit
GGTGGGAGAAAAAAAAAAAAAACGATCGGAAGAGCACACGTCTGAACTCC112.5Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGTGAAAAAAAAAGAAAAAAAAAAAAAAAAATAAAAAGAGCAAAAGACAA112.5No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)