FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n02_retina_93_sample_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n02_retina_93_sample_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28065722
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG4456611.5879192418424153No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1719250.6125800006142724No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTG833600.2970171228803592No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGT418820.149228300629501No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGTCGAGTGCCTCACAGT365340.13017302743895204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1749000.078.483051
AGCAGTG1791150.076.451042
CAGTGGT2001750.068.270244
GCAGTGG2029850.067.3958053
AGTGGTA2031200.067.3266455
GGTATCA2148700.063.647548
GTGGTAT2148050.063.6288766
TGGTATC2186250.062.493697
GTATCAA2960500.046.2802059
AGAGTAC2123400.035.07204418-19
CATGGGG1450900.035.06604424-25
CAACGCA2162250.034.545812-13
ACGCAGA2168950.034.48468814-15
AGTACAT2223600.033.4450220-21
CCTCACT779350.032.8654972-73
TACATGG2255600.032.8085622-23
GCAGAGT2293850.032.59292216-17
ATCAACG2307200.032.48415410-11
AAGGGTT801850.032.4026256-57
CGTTCCT790750.032.38945468-69
Produced by FastQC (version 0.11.2)