FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n02_retina_93_sample_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n02_retina_93_sample_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16133178
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG2267061.4052160088979369No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG862430.5345691964720156No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTG413940.2565768505126516No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGT217040.1345302208901433No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGTCGAGTGCCTCACAGT189360.11737303090562815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT921050.075.5195161
AGCAGTG928200.074.679992
CAGTGGT1025550.067.558734
AGTGGTA1036900.066.822955
GCAGTGG1039100.066.64013
GGTATCA1103050.062.869488
GTGGTAT1106150.062.6793176
TGGTATC1133900.061.0434047
GTATCAA1529450.045.408949
CATGGGG721250.034.82972724-25
AGAGTAC1094450.034.75021418-19
ACGCAGA1115800.034.2095614-15
CAACGCA1114850.034.20502512-13
AGTACAT1136450.033.40221420-21
GCAGAGT1181300.032.31409516-17
CCTCACT404200.032.3064872-73
TGCCTCA409550.032.20043640-41
AAGGGTT411600.032.20034456-57
ATCAACG1188150.032.1933210-11
CGTTCCT406000.032.13795568-69
Produced by FastQC (version 0.11.2)