FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n02_retina_93_sample_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n02_retina_93_sample_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22764744
Sequences flagged as poor quality0
Sequence length99
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG2049570.9003263994534707No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1537880.6755533908046583No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTG395270.17363252580393612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1229200.075.9793241
AGCAGTG1228300.075.7994162
CAGTGGT1363800.068.1741644
GCAGTGG1376350.067.5598453
AGTGGTA1387500.067.060575
GTGGTAT1454950.063.963126
GGTATCA1460100.063.699198
TGGTATC1494400.062.195057
GTATCAA1981150.047.1742679
CATGGGG812100.035.1437424-25
AGAGTAC1462800.034.71665618-19
CAACGCA1480000.034.4121112-13
GCAGAGT1484450.034.2578116-17
ACGCAGA1493050.034.1716914-15
AGTACAT1510750.033.5094620-21
ATCAACG1531350.033.32900210-11
TACATGG1536500.032.85535422-23
CAGAGTA1454350.032.050218-19
CGCAGAG1465100.031.8662816-17
GTACATG1464050.031.61626822-23
Produced by FastQC (version 0.11.2)