FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n02_retina_83_sample_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n02_retina_83_sample_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15499991
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG4168712.689491884221094No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG983590.634574562011036No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTG764780.4934067381071382No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGT388560.25068401652620315No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGTCGAGTGCCTCACAGT357090.23038077893077485No Hit
AGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTGTA164740.10628393268099316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1297950.082.235321
AGCAGTG1337900.079.527472
CAGTGGT1543650.068.847094
AGTGGTA1572900.067.679885
GCAGTGG1579300.067.3279953
GTGGTAT1650450.064.518986
GGTATCA1661100.064.036788
TGGTATC1722650.061.6912737
GTATCAA2429000.043.8961149
CATGGGG1119650.035.28540424-25
AGAGTAC1653650.035.2431218-19
ACGCAGA1664750.035.11808414-15
CAACGCA1671800.034.92187512-13
AGTACAT1688950.034.43895320-21
GCAGAGT1708400.034.2011516-17
ATCAACG1725250.033.8980210-11
AGTGTAT719300.033.3894448-49
AAGGGTT719550.033.3621756-57
TGCCTCA719650.033.21992540-41
CCTCACT717100.033.14321572-73
Produced by FastQC (version 0.11.2)