FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n02_retina_83_sample_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n02_retina_83_sample_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21294564
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG4807022.257393013540921No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1331650.6253473891271031No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTG824470.38717392851997345No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGT428130.20105131055982176No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGTCGAGTGCCTCACAGT422630.19846849177095152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1886150.081.960071
AGCAGTG1914950.080.5936662
CAGTGGT2202000.070.002844
AGTGGTA2232600.069.140835
GCAGTGG2240350.068.893753
GTGGTAT2336100.066.0708546
GGTATCA2336700.065.9638
TGGTATC2421800.063.6461377
GTATCAA3217650.047.915219
CATGGGG1514550.036.22912624-25
AGAGTAC2314850.036.21203218-19
ACGCAGA2335700.035.96237614-15
CAACGCA2348650.035.6970212-13
AGTACAT2370700.035.3219420-21
GCAGAGT2385250.035.19942516-17
ATCAACG2428500.034.6016510-11
CCTCACT808450.034.2946872-73
AAGGGTT820500.034.17328356-57
CGTTCCT812800.033.97683368-69
TCACTAA822050.033.97387374-75
Produced by FastQC (version 0.11.2)