FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n02_retina_83_sample_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n02_retina_83_sample_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25680097
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG3751211.460746040016905No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1808300.7041640068571392No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTG714180.2781064261556333No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGT391260.15235923758387673No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGTCGAGTGCCTCACAGT336010.1308445213427348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1584100.076.405252
AAGCAGT1602100.075.7118451
CAGTGGT1781900.067.896544
AGTGGTA1799400.067.314945
GCAGTGG1833750.066.132263
GTGGTAT1877900.064.578696
GGTATCA1902450.063.6966138
TGGTATC2035250.059.5002987
GTATCAA2628950.046.0810559
AGAGTAC1865600.035.67937518-19
CATGGGG1236400.035.28820824-25
ACGCAGA1901300.035.0607714-15
GCAGAGT1922200.034.73925816-17
CAACGCA1918850.034.7233412-13
AGTACAT1916550.034.5775720-21
ATCAACG1979850.033.69737610-11
CGCAGAG1877550.032.38654716-17
CAGAGTA1876400.032.31804318-19
AACGCAG1891500.032.05376414-15
GTACATG1890650.031.894222-23
Produced by FastQC (version 0.11.2)