FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n01_retina_93_sample_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n01_retina_93_sample_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28065722
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGA121350.019.1350021
CCGGGAT111250.018.464961
AGAGCGA129550.018.3940181
GTTGGAC93650.018.3867848
GGGACCA69650.018.3161133
GAATGAA93900.018.2865471
GGAAGAC54100.018.2044835
GATGAAA79700.018.1317461
TACGGGC104000.018.1263621
TGAGCAT101200.018.1193771
CAATGGA82750.018.0807538
TGGACCC94700.018.0721410
CGCTATC131250.017.9957391
ATAGCGG88600.017.97727410
TGCGTGG80950.017.9425771
CGGGCCA69650.017.9241623
GCCGGAG53600.017.923983
CGGGACC71700.017.894942
TGACGGC58850.017.8813691
GGACCAG65950.017.8469644
Produced by FastQC (version 0.11.2)