FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n01_retina_93_sample_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n01_retina_93_sample_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16133178
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCCG108600.019.5344561
GAGCCGA86850.019.1621822
AAGAAGC93750.018.99438710
TAAGAAG84550.018.8133896
ACGATCG82850.018.6420464
TGACGGC40850.018.5620061
AGAAGAA96650.018.4352058
ACACTAG79450.018.36927
GTTAACG89400.018.356549
TCGTAGG101800.018.3276888
TTAACGA89750.018.32010710
AGCCGAG122100.018.282173
ATAGGAG63150.018.22249810
TGGACGC55500.018.1848681
CAGATAG61550.018.1843817
CAACTGC87450.018.1654918
GGCGACT74750.018.1241761
GGTCCTC73600.018.0888427
CACACTA79750.018.04996
CGATCGT85600.018.0429785
Produced by FastQC (version 0.11.2)