FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n01_retina_93_sample_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n01_retina_93_sample_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22764744
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAATG184200.020.1274051
TGCTACC311250.020.0944041
TGCACCT415650.019.9619051
TGTACGG220550.019.95173510
GGGCATC157550.019.7988831
AGCTGAG206400.019.79854210
ACTGAAC123550.019.798371
GTGTACG222500.019.7721149
TAGTGTA222100.019.7368147
GCTGTAG235050.019.6144493
TGGCTGG172650.019.6063371
ATACATC207100.019.5695193
CACCTCA293550.019.557773
AACACAC284200.019.53608110
TAATAGC189900.019.5062039
CATATTC277550.019.4918481
AGTGTAC243000.019.439228
GTAAGCA185150.019.3828438
CGTAAGC178850.019.3670067
GCTACCT284050.019.365532
Produced by FastQC (version 0.11.2)