FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n01_retina_83_sample_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n01_retina_83_sample_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15499991
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGAG66100.019.0140532
GGGCATC66150.018.4488961
TTGAACG48050.018.4040241
ACGGAGA64650.018.389591
ACACTGA89050.018.3750131
GTGAACG63450.018.318319
TGACGGC42450.018.2588231
GAAGATG38800.018.1306116
TGAGTCG62700.018.10215
CTTAGGC56400.018.0949755
AGTGAAC76200.017.967688
CAATGGA43000.017.9224348
GAGTGAA67850.017.904127
ACTGAAC36150.017.8964371
GGAGGTA95200.017.87791410
GTAAACA63600.017.8623898
ATTGGTG100600.017.8417821
GTCGAGA70550.017.7844458
GCCTGAG39250.017.76230210
CTGTGCT124750.017.7217121
Produced by FastQC (version 0.11.2)