FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n01_retina_83_sample_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n01_retina_83_sample_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21294564
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCCA124900.018.9814915
CCTTCCC176150.018.638581
CAATGGA83650.018.5265398
TCCAAAC160850.018.4078038
GATGCTA89750.018.3960511
TCCCTCC132000.018.3662224
CTCCAAA140250.018.3413377
TGACGGC50400.018.2549251
CCAAACT144750.018.1921889
GATGAAA61650.018.1095091
CAACTAG118750.018.0692961
GGGCATC98500.018.0414161
GGCTCGA66250.017.977681
GACTAAC57800.017.8439141
AGGCGAC23900.017.7922558
GCGCGAT44800.017.7469751
CTGCGGA50800.017.6149881
CGGACTG42450.017.5913181
TGCTACC67700.017.4219841
GGGAATG48500.017.3675731
Produced by FastQC (version 0.11.2)