FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n01_retina_83_sample_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n01_retina_83_sample_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25680097
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC197850.019.8170721
GATGAAA210950.019.8162331
GTCGGGA155300.019.6873747
AGGCGAA106800.019.63264810
GCAGGCG190650.019.5946257
AGCAAAC194000.019.5539326
TAACGCA169050.019.3783762
TGAGCCG107500.019.3550171
GGCAGGC194750.019.3438456
CTAATAG176100.019.3179533
ACGCAGA169150.019.1618544
TGAGCAT166950.019.118851
GCAAACG164550.019.0847457
ATAGCTA174950.019.0785086
AACGCAG172100.019.0714443
TTGGCAA203750.019.0628071
AAAGTTG172300.019.0125185
TGACGGC77550.018.961111
AACGTGG176550.018.88200210
CCGTACT221750.018.8747941
Produced by FastQC (version 0.11.2)