FastQCFastQC Report
Fri 10 Aug 2018
HGMTYBGX7_n03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMTYBGX7_n03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303143112
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40216501.3266506283012625No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAACCAACCGGGA4027300.13285144344628882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG6944850.082.085112
AAGCAGT7443000.076.696381
GCAGTGG8332800.068.669133
CAGTGGT8623250.066.2440954
AGTGGTA9932000.057.571595
GTGGTAT12095600.047.468686
GTATCAA12506200.045.7302179
TGGTATC15383350.037.2912377
CATGGGG4854300.033.9018424-25
CAACGCA8925450.033.85688412-13
AGAGTAC9086550.033.29785518-19
TACATGG9034250.033.26685722-23
GGTATCA17659950.032.8887068
CGCAGAG8743850.032.73383316-17
AGTACAT9295900.032.3965420-21
ACGCAGA9375600.032.33611314-15
CAGAGTA8968800.031.76639218-19
GCAGAGT9579900.031.63146216-17
GTACATG8975300.031.55229422-23
AACGCAG9122250.031.24037214-15
Produced by FastQC (version 0.11.2)