FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35906577
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8210442.2866117257570946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA457050.019.8426512
CGGAAGA476200.019.3332754
TCGGAAG479950.018.8074953
GATCGGA474000.018.7027911
GAGCGTC457050.018.3311129
TCGTGTA241050.018.066214-15
AGAGCGT491700.017.1927228
AAGAGCG550150.016.0051637
GTAGGGA254500.015.18006918-19
TAGGGAA240800.014.9787418-19
AGCGTCG319400.013.76128910-11
CGTGTAT45800.013.37607914-15
GAAGAGC733750.012.7835576
GTGTAGG308700.012.15324716-17
TGTAGGG297100.012.02034816-17
AGGGAAA369950.012.01543620-21
GGAAGAG831050.011.828875
GGGAAAA419800.010.54333722-23
AAAGGGG351700.010.46628680-81
GGAAAAA475600.010.44965622-23
Produced by FastQC (version 0.11.5)