FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n02_HFHm16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n02_HFHm16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38108743
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG594600.012.7098380-81
AAAAGGG601400.012.40025780-81
TAGGGGG215900.012.16591450-51
GGGAAAA549050.011.68332222-23
AAGGGGG630100.011.65836782-83
TAAAAAA1350900.011.52368470-71
AAAAAGG816100.011.34393778-79
GGGGGGA953450.010.4586032
CGCGAAT18400.010.0782271
AAAAAAG1197850.09.53096878-79
GGGGGAA602150.09.17788920-21
CGGGGAT124600.09.1204691
GGGTAAA210050.08.85304622-23
AGGGGGG1016000.08.84554982-83
GGGGGAG857450.08.6732633
TATAGGG91850.08.63612648-49
CCGGGAT119400.08.6017491
CGGGAAT92150.08.5654491
GGGGGGT940500.08.43647614-15
AAAAAAA8113750.08.07921774-75
Produced by FastQC (version 0.11.5)