FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n02_HFHm15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n02_HFHm15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33502641
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG500400.012.54432780-81
AAAAGGG506200.011.38715280-81
GGGAAAA476550.011.0187822-23
AAAAAGG700950.010.98498278-79
TAAAAAA1149550.010.97786970-71
TAGGGGG185100.010.9699350-51
AAGGGGG532450.010.74998982-83
GGGGGGA791000.010.642082
CGCGAAT16000.010.1033821
CGGGGAT110750.09.6593221
CCGGGAT104900.09.110211
GGGGGAA498250.09.01837620-21
GGGGGAG734800.08.7309633
AAAAAAG1034750.08.55914378-79
GGGTAAA183150.08.51945322-23
AGGGGGG882000.08.44994982-83
CGGGTAT40700.08.2941481
CGCGAGT37300.08.2853781
CGGGAAT76850.08.1046681
CGGGAAC127000.07.97413731
Produced by FastQC (version 0.11.5)