FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n02_HFHm14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n02_HFHm14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37177561
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG547250.012.468472580-81
GGGGGGA823700.012.4059132
AAGGGGG551600.012.18500182-83
GGGAAAA540600.012.06362922-23
AAAGGGG537550.011.83191780-81
TAGGGGG182500.010.91807750-51
TAAAAAA1316700.010.55675870-71
GGGGGAG756950.010.2701143
AAAAAGG764500.010.06235178-79
AAAAAAG1138500.09.34568278-79
GGGTAAA209950.09.23055422-23
GGGGGGT766400.08.90288414-15
AATCGTA32600.08.8852655
TATAGGG89950.08.87142448-49
GGGGAGA520700.08.699834
GGGGGAA550050.08.62236620-21
CCGGGAT115950.08.6109921
CGGGGAT121600.08.6018891
AGGGGGG840300.08.53281182-83
CGGGGGG562050.08.4085112-13
Produced by FastQC (version 0.11.5)