FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n02_HFHm13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n02_HFHm13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36431578
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG562300.013.27112980-81
AAAAGGG561950.012.00724380-81
AAAAAGG764100.011.59082478-79
AAGGGGG590600.011.54542282-83
TAGGGGG207050.011.35531850-51
TAAAAAA1279450.011.20739970-71
GGGAAAA528950.011.0989122-23
GGGGGGA890700.010.0277672
AGGGGGG963750.09.172379582-83
GGGGGAA565500.09.16367720-21
AAAAAAG1139450.08.67727478-79
GGGGGAG808900.08.6654623
GGGTAAA202050.08.54519622-23
CGGGAAT87000.08.3071461
CGGGGAT121400.08.0681891
CGGGGGG646450.08.05639812-13
CCGGGAT113750.07.9837971
GTCGGTT60950.07.8790731
AAAAAAA7610300.07.8540974-75
GGGGAAT245350.07.821890462-63
Produced by FastQC (version 0.11.5)