FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n02_HFHm12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n02_HFHm12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32521315
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG587850.015.09015480-81
AAAAGGG592150.013.452435580-81
AAAAAGG783250.013.22985478-79
AAGGGGG641700.012.55797982-83
GGGAAAA488800.012.511168522-23
TAAAAAA1257600.012.15178870-71
TAGGGGG198300.011.06609550-51
GGGGGAA566600.010.05144420-21
AAAAAAG1128750.09.93149178-79
GGGGGGA987700.09.6534972
AGGGGGG1090350.09.58635182-83
GGGTAAA192250.09.03031422-23
CGGGAAT80900.08.9922881
GGGGAAA516550.08.83675722-23
GGGGGAG841500.08.7584043
AAAAAAA7460750.08.72996774-75
CGGGGAT110550.08.5159631
AATAGGG126650.08.38246880-81
GGGGGAT293950.08.2333762-63
GGGGGGT979550.08.18277114-15
Produced by FastQC (version 0.11.5)