FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n02_HFHm11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n02_HFHm11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32585063
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG561550.015.53879380-81
GGGAAAA523800.014.3093222-23
AAAAGGG582900.014.12623180-81
AAGGGGG604000.013.72718382-83
TAGGGGG218350.013.46524650-51
AAAAAGG794150.013.35332678-79
TAAAAAA1414500.013.17331970-71
GGGTAAA210800.012.19001322-23
GGGGGGA1028700.012.0763072
GGGGGAG850650.010.9587053
GGGGGAA579150.010.79717920-21
AAAAAAG1203100.010.57129778-79
AGGGGGG1048300.09.93918182-83
GGGGAGA516550.09.7532254
CGGGGGG654450.09.47486312-13
GGTAAAA262800.09.38939822-23
GGGGAAT239000.09.2421262-63
GGGGTAA186000.09.14219820-21
CGGGAAT73900.09.0069621
GGGGAAA548400.08.938422-23
Produced by FastQC (version 0.11.5)