FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n02_HFHm10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n02_HFHm10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30191487
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG768960.25469431167799056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG370700.08.87348178-79
AGGGGGG873450.08.71469480-81
AAGGGGG425900.08.59882280-81
CCGGGAT97950.08.4946291
CGGGGAT106950.08.0909841
CGCGAAT16101.8189894E-127.97349451
AAAAGGG344700.07.69624378-79
CGCGTAT13004.3746695E-97.68045141
CGGGCAT112400.07.61407231
CGGGAAT73750.07.60730361
GGGGTTA125550.07.586189366-67
GGGAAAT233550.07.4916711
CGAATCG18951.8189894E-127.2674853
CGCGAGT38800.07.22987941
GGGGGTT437100.07.04231664-65
GGGGAAT179050.06.9572521
CTCGGAT87250.06.92068431
CGGGAAC113950.06.8429011
GAATCGC54150.06.8399914
CTCGTAT37000.06.8105931
Produced by FastQC (version 0.11.5)