FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n02_HFHm09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n02_HFHm09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33159051
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAAAA646600.017.20783822-23
AAAGGGG722750.017.15007680-81
AAAAGGG734850.016.30531980-81
TAGGGGG253750.016.1449450-51
AAAAAGG960800.015.31116878-79
AAGGGGG790900.015.09273982-83
TAAAAAA1693650.014.3724670-71
TATAGGG102450.014.09463648-49
GGGGGAG1005950.012.7221363
AAAAAAG1427300.012.61649278-79
GGGGGGA1337150.012.5979912
GGGTAAA240550.012.3708122-23
GGGGGAA736800.012.29050320-21
GGGGAGA586750.011.7102514
GGGGAAT274350.011.193747562-63
CGGGGGG770550.010.70694712-13
AGGGGGG1345350.010.56741182-83
GGGGAAA679100.010.50904322-23
AAATGGG360750.010.31643280-81
GAGCGGG271600.010.2825429
Produced by FastQC (version 0.11.5)