FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n02_HFHm08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n02_HFHm08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34935465
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG480650.011.90844180-81
TAGGGGG173750.010.21041450-51
AAAAAGG681300.09.7818178-79
TAAAAAA1109550.09.48355770-71
AAGGGGG490300.09.22788382-83
CGGGAAT81450.09.2231
AGGGGGG779550.09.18874882-83
AAAAGGG483350.09.00673980-81
CGGGCAT123800.08.9099151
CCGGGAT108750.08.6127761
CGGGGAT111600.08.3928261
CGGGATT68350.08.0691111
GGGAAAA442050.07.73106722-23
CGGCAAT70050.07.6696671
GGGGGAA468100.07.64077320-21
GGGGGGA720250.07.5983212
AAAAAAG992050.07.417028476-77
GCGTCGT271400.07.281159494-95
GATCGGA640400.07.254451394-95
CGCCAAT61000.07.24868631
Produced by FastQC (version 0.11.5)