FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n02_HFHm07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n02_HFHm07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33681741
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG404800.010.90118880-81
AAAAGGG396200.09.77108478-79
CGGGAAT80250.09.1825051
CGCGAAT14800.08.6731441
CTCGGAT98400.08.0685581
CGGGGAT107400.07.92361261
CCGGGAT102700.07.8696051
GGCGAAT47350.07.8315751
AGGGGGG694100.07.82210282-83
AAAGGGG387700.07.79221578-79
CGACGAT41300.07.4823461
CGGAAAT73750.07.47774171
GGGAAAT266050.07.3264751
AGCGTCG300850.07.32628394-95
GGGGAAT188850.07.30054861
GCGTCGT260900.07.291957494-95
CGCGGAT50300.07.27775141
GATCGGA621050.07.23569594-95
CGGGGTT117750.07.22714231
CCGGAAT66400.07.1598911
Produced by FastQC (version 0.11.5)