FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n02_HFHm06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n02_HFHm06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37918719
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG484550.011.4106980-81
TAGGGGG168350.011.0457350-51
AGGGGGG720800.09.31818482-83
CGGGGAT118400.09.195721
CGCGGAT55850.09.1939581
AAGGGGG494600.09.11879682-83
CGCGAAT16700.09.1103581
AAAAAGG713950.09.10821878-79
CCGGGAT120950.09.0411551
TAAAAAA1166050.08.89576770-71
AAAAGGG500700.08.87016280-81
CGGGAAT91350.08.6397551
CGCGGGT83200.08.1717421
GGGAAAA477650.08.02993422-23
GGGGGAA471900.07.93156520-21
CTCGGGT107500.07.73983141
GGGGGGA692500.07.7239282
GGGGGGT691450.07.480774414-15
TATAGGG85600.07.463294548-49
CTCGGAT105000.07.38074541
Produced by FastQC (version 0.11.5)