FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n02_HFHm05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n02_HFHm05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32858684
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG473750.013.04933680-81
AAAAAGG668450.010.730165578-79
TAGGGGG163450.010.43251850-51
AGGGGGG788750.09.84025882-83
TAAAAAA1084350.09.83958370-71
AAGGGGG492600.09.65718182-83
AAAAGGG484550.09.4107980-81
CGGGGAT106100.09.2314641
CCGGGAT98100.08.5787321
GGGAAAA428950.08.54304822-23
GGGGGAA459800.08.39850220-21
CGCGAAT14509.094947E-128.1976771
GGGGGGA721050.08.1437152
AAAAAAG986050.08.13420476-77
CTCGTAT37150.07.93508671
CGGGAAT80750.07.47790581
CTCGGGT100050.07.36602931
GATCGGA540250.07.333154794-95
CTCGGAT91100.07.30682041
AAAAAAA5940450.07.27099474-75
Produced by FastQC (version 0.11.5)