FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n02_HFHm04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n02_HFHm04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35118480
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG484550.012.77349880-81
AAAAAGG697900.010.35227378-79
AAGGGGG519200.09.74822682-83
AAAAGGG502550.09.56072180-81
TAAAAAA1131450.09.24312770-71
AGGGGGG852600.09.15096482-83
TAGGGGG173200.08.50142250-51
CGCGGAT56600.08.4002111
CGGGGAT114400.08.0627351
GGGGGAA491350.08.05261720-21
CCGGGAT106400.07.90930371
GGGAAAA461950.07.72710322-23
CGGGAAT85300.07.6362151
TACCGTA25850.07.53316649
CGCGAAT15252.2009772E-107.48252251
AAAAAAG1035050.07.434723476-77
GGGGGGA773600.07.43030552
CGGGAAC128100.07.3489061
CGGGCAT125900.07.32626531
GGGAAAT291650.07.156641
Produced by FastQC (version 0.11.5)