FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n02_HFHm03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n02_HFHm03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29693129
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG399250.012.99772680-81
AAAAAGG574200.010.48531578-79
CGGGAAT71550.010.36681651
AGGGGGG657700.010.15784682-83
TAAAAAA968350.09.77725770-71
AAAAGGG404150.09.6199578-79
AAGGGGG404950.09.25478780-81
CGCGGAT47300.08.9466271
TAGGGGG148350.08.90087550-51
AAAAAAG868850.08.15985376-77
GGGGGGA632250.08.0401832
GGGGGAA391350.07.961991320-21
CGGGCAT105650.07.7408631
GGGAAAA368600.07.602877622-23
CGAGAAT59450.07.59805061
CCGGGAT90300.07.58237361
GGGGGAT228400.07.53917862-63
CGGGGAT93300.07.33856731
AAAAAAA5181900.07.148676474-75
TACCGTA21450.07.08552749
Produced by FastQC (version 0.11.5)