FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n02_HFHm02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n02_HFHm02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27631231
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG417540.15111161714076365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAAAT206300.07.60547261
CGGGGAT115550.07.52995871
AATCGAA42200.07.313935
CCGGAAT61250.07.21918771
GGGGAAT160700.07.1895531
CGGGAAT74050.07.0628361
CTCGGAT84750.06.9565431
AAATCGA58150.06.9406774
CGCGAAT19351.2551027E-106.63428741
TCGGATC49200.06.56576822
GGAAAAT260350.06.55614231
GAAATCG71950.06.5340883
CCGGGAT104850.06.52988531
GGCGAAT50000.06.46621941
GGGAGAT251650.06.3671411
CGGGAAC107850.06.30416251
CAATCCA131900.06.2997874
AATTGAA166700.06.29515745
CGGAATC59850.06.27053642
GGAGAAT272600.06.26152471
Produced by FastQC (version 0.11.5)