FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n02_HFHm01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n02_HFHm01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32513780
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG422900.011.48473780-81
AAAAGGG417000.010.25761578-79
AAGGGGG428400.010.05110580-81
AGGGGGG674200.010.03621982-83
TAGGGGG153450.09.73494450-51
CGCGAAT14900.09.2540021
AAAAAGG601200.08.89647978-79
CCGGGAT103500.08.728331
GGCGAAT48600.08.3157331
CGGGGAT102450.08.2608721
TAAAAAA977800.08.03564970-71
CGCGGAT49300.07.9083311
CGAATCG21050.07.8960613
GCGAATC41550.07.8891392
AAAAAAG901900.07.763350576-77
CGGGGTT107500.07.6958871
GGGAAAT256500.07.48879431
GCGGTAT33950.07.42256451
CTCGGGT96900.07.3110611
GGGGGGA625750.07.26432320-21
Produced by FastQC (version 0.11.5)