FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35906577
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8741402.434484356445339No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT3716931.0351668999247685TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT2603980.7252097575327217TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC1935550.066.328618
CGGAAGA2054350.063.079164
TCGGAAG2062200.062.8088383
GAGCACA2054500.062.2685789
ATCGGAA2080100.062.086872
GATCGGA2011450.060.315581
AAGAGCA2200800.058.841717
GAAGAGC2273250.056.838516
GGAAGAG2389250.054.370955
AATCGGA75750.049.1807021
TATCGGA42100.045.883821
TCTCGTA951800.043.8893342-43
CTCGTAT967700.043.722544-45
TATCTCG574450.043.530140-41
CGTATCT549550.043.4620538-39
ATGCCGT1071500.043.44954748-49
ATCTCGT967700.042.54968642-43
TCGTATC576100.042.37433238-39
CGAATTC678950.041.3068832-33
GTCACGA705150.041.18963628-29
Produced by FastQC (version 0.11.5)