FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n01_HFHm16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n01_HFHm16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38108743
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT5923191.554286374651612TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC1420250.050.7921078
GAGCACA1420900.050.762069
TCGGAAG1507050.047.8835833
ATCGGAA1511250.047.5468862
CGGAAGA1519700.047.5227554
GATCGGA1540550.045.8129161
AAGAGCA1591650.045.4778257
CGTATGC757700.044.6824546-47
TATGCCG761450.044.4930348-49
CTCGTAT733650.044.1246344-45
TCGTATC742100.043.97915338-39
TCGTATG760300.043.95559344-45
CGTATCT726750.043.845938-39
TATCTCG729000.043.49882540-41
ATTCGTA817150.042.9017536-37
TCTCGTA740400.042.7223442-43
TTCGTAT800800.042.5823936-37
AATTCGT837250.042.10165834-35
ACGAATT847500.042.0852332-33
GAAGAGC1730250.041.9212346
Produced by FastQC (version 0.11.5)