FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n01_HFHm15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n01_HFHm15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33502641
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT4762841.421631208118787TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC1145800.051.4503638
GAGCACA1149150.051.296179
TCGGAAG1175700.050.219863
ATCGGAA1173550.050.029132
CGGAAGA1201750.049.0643584
GATCGGA1189050.048.598821
AAGAGCA1291250.045.747027
CGTATGC616750.044.5889246-47
TATGCCG624250.044.0566548-49
TCGTATG619400.043.86586444-45
TCGTATC603300.043.81763538-39
CTCGTAT598200.043.7846844-45
CGTATCT590750.043.5825538-39
TATCTCG591600.043.3474640-41
ATTCGTA663950.043.0164836-37
TTCGTAT646500.042.75211736-37
GAAGAGC1400000.042.2556656
TCTCGTA605450.042.21375342-43
ACGAATT690050.042.1952232-33
AATTCGT683350.041.97693334-35
Produced by FastQC (version 0.11.5)