FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n01_HFHm14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n01_HFHm14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37177561
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT5055061.359707270737852TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC1236250.050.8050848
GAGCACA1245750.050.5054139
TCGGAAG1280550.049.0976453
ATCGGAA1279450.048.9288982
CGGAAGA1309950.047.977974
GATCGGA1295000.047.1035461
AAGAGCA1397850.045.1426857
CGTATGC662200.044.32996446-47
TATGCCG666800.044.02712248-49
TCGTATG665550.043.62634744-45
CTCGTAT641800.043.5717544-45
TCGTATC644600.043.46503438-39
CGTATCT635400.043.18975438-39
TATCTCG635750.043.0203640-41
ATTCGTA713400.042.56270636-37
TTCGTAT696550.042.31701736-37
TCTCGTA647400.042.0984642-43
AATTCGT729350.041.99008634-35
ACGAATT745950.041.5102532-33
GAAGAGC1524500.041.450156
Produced by FastQC (version 0.11.5)