FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n01_HFHm13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n01_HFHm13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36431578
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT5403431.4831720986667116TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1146400.057.4248922
TCGGAAG1168500.056.5738833
GATCGGA1152100.056.2700461
AGAGCAC1184450.055.53458
GAGCACA1193950.055.032579
CGGAAGA1204150.054.737684
AAGAGCA1327200.049.715467
GAAGAGC1442050.045.974546
CGTATGC694400.044.5010346-47
TATGCCG696550.044.27145448-49
TCGTATC677900.043.97377438-39
CTCGTAT672550.043.90260344-45
TCGTATG696900.043.81022644-45
CGTATCT666550.043.65001338-39
TATCTCG667050.043.3681740-41
ATTCGTA747950.042.89740836-37
TTCGTAT728600.042.75882336-37
TCTCGTA679300.042.45967542-43
ACGAATT769900.042.39056832-33
AATTCGT762050.042.34478834-35
Produced by FastQC (version 0.11.5)