FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n01_HFHm12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n01_HFHm12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32521315
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT6181941.900888694076485TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC1430300.051.714078
GAGCACA1434150.051.660969
TCGGAAG1481600.050.0373883
ATCGGAA1479700.050.009082
CGGAAGA1499600.049.3514064
GATCGGA1488150.048.8860441
AAGAGCA1581350.046.886157
CGTATGC777950.044.94214246-47
TATGCCG780200.044.82097648-49
CTCGTAT753300.044.27295744-45
TCGTATC760550.044.14843838-39
TCGTATG780100.044.12203644-45
GAAGAGC1689400.043.9702456
CGTATCT745900.043.94882238-39
TATCTCG744950.043.79139340-41
TCTCGTA754750.043.18137442-43
ATTCGTA835100.043.17663236-37
TTCGTAT812100.042.96637336-37
AATTCGT858250.042.1508134-35
ACGAATT873400.042.060732-33
Produced by FastQC (version 0.11.5)