FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n01_HFHm11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n01_HFHm11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32585063
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT7106222.180821316810098TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1912750.045.833939
AGAGCAC1940900.045.140018
TATGCCG912900.045.02840448-49
CGTATGC914050.044.99821546-47
CTCGTAT879450.044.31169544-45
TCGTATG922650.043.92302744-45
TATCTCG868850.043.8237640-41
TCGTATC894400.043.77750838-39
CGTATCT876650.043.62891438-39
TCTCGTA873150.043.457242-43
TTCGTAT964600.042.56633436-37
ATGCCGT958350.042.45427748-49
ATTCGTA1000450.042.44885336-37
ATCTCGT896800.042.43034442-43
CGGAAGA2096950.041.949584
GTATGCC980800.041.91399446-47
AAGAGCA2103550.041.735587
AATTCGT1024350.041.70585634-35
TCGGAAG2112850.041.6493153
ATCGGAA2119350.041.284382
Produced by FastQC (version 0.11.5)