FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n01_HFHm10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n01_HFHm10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30191487
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT5703271.889032494490914TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1517350.045.0592659
CGTATGC719650.044.9660146-47
TATGCCG721500.044.8994248-49
AGAGCAC1528900.044.777978
TCGTATG720200.044.47387344-45
CTCGTAT698700.044.28893744-45
TATCTCG695300.043.6907140-41
TCGTATC713650.043.6821938-39
CGTATCT700850.043.62597338-39
TCTCGTA704100.043.11002742-43
TTCGTAT767600.042.37568736-37
ATTCGTA791050.042.27546736-37
ATCTCGT721200.042.20971342-43
TCGGAAG1643750.041.841763
CGGAAGA1645600.041.789154
ATGCCGT772150.041.64353648-49
ATCGGAA1647700.041.5835082
AATTCGT810800.041.447934-35
GTATGCC788650.041.06500246-47
AAGAGCA1672550.041.0459337
Produced by FastQC (version 0.11.5)