FastQCFastQC Report
Sat 1 Jun 2019
HGMN5BGXB_n01_HFHm09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMN5BGXB_n01_HFHm09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33159051
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT9457962.8523011711040827TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2155450.053.655259
AGAGCAC2185150.052.984678
CGGAAGA2341100.049.507894
TCGGAAG2346150.049.421053
AAGAGCA2347250.049.412597
ATCGGAA2360400.048.9443242
GATCGGA2380550.047.541521
GAAGAGC2488000.046.722596
CGTATGC1188600.045.52477346-47
TATGCCG1189850.045.50623348-49
CTCGTAT1144800.044.9931544-45
CGTATCT1138900.044.6664738-39
TATCTCG1131950.044.6595840-41
TCGTATG1196600.044.61345744-45
TCGTATC1166200.044.54142438-39
TCTCGTA1139950.044.22451842-43
ATTCGTA1276850.043.7415436-37
TTCGTAT1241250.043.70063836-37
ATCTCGT1159200.043.5905142-43
ATGCCGT1236200.043.48110648-49
Produced by FastQC (version 0.11.5)